Protocols

The protocols are organized into following categories. The pages for protocols will be hyperlinked so that some of the protocols may appear in different places. Grayed out sections are still under construction.

All About Media

• Background- Media for bacteria
  
  • Media Recipes for Pseudomonas and Salmonella

  Molecular Biology and cloning

• • Gibson cloning

• General Bacteriology
  • Competent Cell Preparation Protocol
  • Transformation
  • Conjugation
  • Transduction
  • Antibiotic resistance determination
  • Growth curves, growth rate calculations

• Bacterial phenotyping
  • Pseudomonas phenotypes

• Bacterial genetics
  • Lambda Red Recombineering for Salmonella
  • P22 Transduction Protocol
  • Conjugating plasmids into bacteria
  • Mariner Transposon Mutagenesis
  • Tn10 mutagenesis protocol for Salmonella

• Protein Purification
  • Test Expression
  • Protein expression tips
  • Bradford Assay
  • 2D Gel Protocol
  • Size Exclusion Chromatography on Acta FPLC
  • Nickle Affinity Chrmatography on Acta FPLC
  • High throughput protocol for Protein Refolding
  • Thrombin Cleavage of Protein tags
  • Stripping and cleaning Hi Trap columns- Complete protocols

Instrument manuals and  protocols(Janus Robot, Biotek Microflo, Envision Plate reader, Horiba Jobin Florometer, Qpix2, Acta FPLC, Shimadzu HPLC, Xena, IVIS, BD Aria, Guava, Ultracentrifuge)

 

Inner and outer membranes

• Making spheroplasts