Anu Chaudhary

 

I am a Research Assistant Professor in Microbiology at the University of Washington, and an Affiliate Scientist at the Los Alamos National Laboratory. I currently work at the Northwest Regional Center for Excellence on topics relating to bacterial pathogenesis and innate immunity. Earlier, I was a principal investigator in the Biosecurity and Public Health Group in the Biosciences Division at Los Alamos National Laboratory (2002-2011).

I received my Ph.D. in Bio-organic Chemistry from SUNY-Stony Brook in 1997 under the guidance of Dr. Glenn D. Prestwich in synthesis and biochemical characterization of photoactivatable analogs of phosphatidylinositol polyphosphates.  Subsequently, from 1997-1999, I pursued postdoctoral studies in Cell Biology at Harvard Medical School in the laboratory of Dr. Joan S. Brugge, developing modified nucleotide analogs and engineered kinases in order to identify direct substrates of protein kinase Akt.  In parallel, I studied integrin-mediated cell signaling and identified a role for protein kinase Pak in phosphatidylinositol 3’- kinase mediated Raf signaling.  I joined the Fred Hutchinson Cancer Research Center as a postdoctoral associate in Dr. Jonathan A. Cooper’s laboratory in 1999 and continued to employ chemical methods to probe direct substrates of tyrosine kinase c-Src.   From 2001-2002, I was a Staff Scientist at the Metabolic and Respiratory Diseases Group in Wyeth Research, Cambridge and worked on developing assays for high throughput screening of protein kinase C isoforms.

Research Interests

  • Protein-Protein Interactions: i) Interactions mediated by the host in response to detection of pathogen components by innate immune receptors (toll like receptors). Using split-Gaussia luciferase and Geoff Waldo’s split-GFP approaches, we have developed cell-based assays to study adaptor protein (MyD88, Mal-TIRAP, TRAM, TRIF) homo- and hetero-dimerizations. ii) Interactions mediated by pathogens in the host to evade detection by the host. Using protein complementation assays, we have analyzed the interactions of Brucella melitensis TIR-like protein (TcpB) with host adaptor proteins, and find that TcpB interacts with the DD of MyD88.
  • Genetic factors that determine susceptibility to infection. I am working with Dennis Ko on elucidating mechanism by which specific SNPs alter Salmonella typhimurium infection in vitro.
  • Publications

    A.M. Dandekar, H. Gouran, A.M. Ibáñez, S.L. Uratsu, C. B. Agüero, S. McFarland, Y. Borhani, P. A. Feldstein, G. E. Bruening, R. Nascimento, L.R. Goulart, P.E. Pardington, A. Chaudhary, M. Norvell, E. Civerolo, G. Gupta, ” An Engineered Innate Immune Defense Protects Grapevines from Pierce’s Disease”, Proc. Natl. Acad. Sci. In press (2012).

    A. Chaudhary, K. Ganguly, S. Cabantous, G.S. Waldo, S. -N. Micheva-Viteva, K. Nag, W.S. Hlavacek, C. -S. Tung, “The Brucella TIR-like Protein TcpB Interacts With The Death Domain of MyD88”, Biochem. Biophys. Res. Comm. 417:299-304 (2012).

    A. Zeytun, A. Chaudhary, P. Pardington, R. B. Cary, G. Gupta, “Induction of Cytokines and Chemokines by Toll-like Receptor Signaling: Strategies for Control of Inflammation”. Crit. Rev. Immunol. 30:1 (2010).

    S. Silva, K. Ganguly, T.M. Fresquez, G. Gupta, T.M. McCleskey, A. Chaudhary , “Beryllium Alters LPS-Mediated STAT3 Phosphorylation and Cytokine Release in Human Peripheral Blood Mononuclear Cells”. J. Occup. Environ. Hyg. 6:775-782 (2009).

    H.V.K. Diyablanage, K. Ganguly, G. E. Collins, B.L. Scott, A. Chaudhary, A. K. Burrell, T. M. McCleskey, “Three Coordinate Ligands for Physiological Beryllium Imaging by Fluorescence”.Angew. Chem. Int. Ed. 120, 7442-7444 (2008).

    A. Chaudhary, T.M. Fresquez and M.J. Naranjo, “Tyrosine Kinase Syk Associates with Toll Like Receptor 4 and Regulates Signaling in Human Monocytic Cells”. Immunol. Cell Biol. 85: 249-256 (2007).

    A. Chaudhary, N.N. Sauer and G. Gupta,Beryllium-Mediated Immune Response in Primary Cells from Healthy Individuals” Toxicol. 201: 9-19 (2004).

    A. Chaudhary, J.S. Brugge and J.A. Cooper, “Direct Phosphorylation of Focal Adhesion Kinase by c-Src:  Evidence Using a Modified Nucleotide Pocket Kinase and ATP-Analog” Biochem. Biophys. Res. Comm. 294: 293-300 (2002).

    X. Li,  M.P. Rivas, M. Fang, J. Marchena, B. Mehrotra, A. Chaudhary, L. Feng, G.D. Prestwich and V. A. Bankaitis, “Analysis of Oxysterol Binding Protein Homologue Kes1pFunction in Regulation of Sec14p-Dependent Protein Transport from the Yeast Golgi Complex” J Cell Biol. 157(1):63-77 (2002).

    G.S. Kular, A. Chaudhary, G.D. Prestwich, P. Swigart, S. Cockcroft and R. Wetzker, “Co-operation of Phosphatidylinositol Transfer Protein with Phosphoinositide 3-Kinase g in the Generation of 3-Phosphorylated Inositol Lipids,” Adv. Enzyme Reg. 42: 53-61 (2002).

    A. Chaudhary, W.G. King, M.D. Mattaliano, J.A. Frost,  B. Diaz, D.K. Morrison, M.H. Cobb, M.S. Marshall and J.S. Brugge, “Phosphatidylinositol 3’- Kinase Regulates Raf-1 through Pak Phosphorylation of Serine 338″ Curr. Biol.10: 551-554 (2000).

    A.A. Profit, J. Chen, Q.-M. Gu, A. Chaudhary, K. Prasad, E.M. Lafer and G.D. Prestwich, “Probing the Phosphoinositide Binding Site of the Clathrin Assembly Protein AP-2 with Photoaffinity Labels” Arch.Biochem. Biophys. 357: 85-94 (1998).

    A. Chaudhary, J. Chen, Q.-M. Gu, W. Witke, D.J. Kwiatkowski and G.D. Prestwich, “Probing the Phosphoinositide 4,5 Bisphosphate Binding Site of Human Profilin I” Chem. & Biol. 5: 273-281 (1998).

    K.M. Loyet, J.A. Kowalchyk, A. Chaudhary, J. Chen, G.D. Prestwich, T.F.J. Martin, “The Specific Binding of Phosphatidylinositol 4,5 Bisphosphate to Calcium-Dependent Activator Protein for Secretion (CAPS), a Potential Phosphoinositide Effector Protein for Regulated Exocytosis” J. Biol. Chem. 273: 8337-8343 (1998).

    A. Chaudhary, Q.-M. Gu, O. Thum, A.A. Profit, Y. Qing, L. Jeyakumar, S. Fleischer and G.D. Prestwich, “Specific Interaction of Golgi Coatomer aCOP with Phosphatidylinositol 3,4,5 Trisphosphate” J. Biol. Chem. 273: 8344-8350 (1998).

    A. Chaudhary and G.D. Prestwich, “A Photoaffinity Analogue for the Anti-inflammatory Drug- a-Trinositol:  Synthesis and Identification of Putative Molecular Targets” Bioconjugate Chem.8: 680-685 (1997).

    A. Chaudhary, B. Mehrotra and G.D. Prestwich, “Rapid Purification of Reporter Group-Tagged-Inositol Hexakisphosphate on Ion Exchange Membrane Adsorbers” BioTech. 23: 427-430 (1997).

    E. Tall, G. Dorman, P. Garcia, L. Runnels, S. Shah, J. Chen, A.A. Profit, Q-M. Gu, A. Chaudhary, G.D. Prestwich and M.J. Rebecchi, “Phosphoinositide Binding Specificity among Phospholipase C Isozymes as Determined by Photo-Cross-Linking to Novel Substrate and Product Analogs” Biochem. 36: 7239-7248 (1997).

    B. Mehrotra, J.T. Elliott, J. Chen, J.D. Olszewski, A.A. Profit, A. Chaudhary, M. Fukuda, K. Mikoshiba and G.D. Prestwich “Selective Photoaffinity Labeling of the Inositol Poly phosphate Binding C2B Domains of Synaptotagmins” J. Biol. Chem. 272: 4237-4244 (1997).

    A. Chaudhary, G. Dorman and G.D. Prestwich, “Synthesis of P-5 Tethered Inositol 1,2,6 Trisphosphate, An Affinity Reagent for a-Trinositol Receptors” Tetrahedron Lett. 35: 7521-7524 (1994).

    Book Chapters and Invited Reviews

    K. Nag and A. Chaudhary, “Mediators of Tyrosine Phosphorylation in Innate Immunity: from Host Defense to Inflammation onto Oncogenesis” Current Signal Transduction Therapy, 4(2), 76-81 (2009).

    B. L. Scott, T.M. McCleskey, A. Chaudhary, E. Hong-Geller and S. Gnanakaran, “ The Bioinorganic Chemistry and Associated Immunology of Chronic Beryllium Disease”  Feature Article.Chem. Comm. 2837-2847 (2008).

    E. Hong-Geller, A. Chaudhary and S. Lauer, “Targeting Toll-Like Receptor Signaling Pathways for Design of Novel Immune Therapeutics” Curr. Drug. Discovery Technol. 5(1), 29-38 (2008).

    E. Hong-Geller and A. Chaudhary, “Chemokine Function in Granulomatous Lung Disease”, Chapter 4 in Chemokine Research, Nova Science Publishers, New York. ISBN: 978-1-60021-641-1, 73-84 (2007).

    G. D. Prestwich, A. Chaudhary, J. Chen, L. Feng, B. Mehrotra and J. Peng, “Probing Phosphoinositide Polyphosphate Binding to Proteins” Symposium Series No. 718, Chapter 2 in Phosphoinositides:  Chemistry, Biochemistry, and Biomedical Applications ISBN: 0-8412-3628-3 (1997).

    G. D. Prestwich, G. Dorman, J.T. Elliott, D.M. Marecak and A. Chaudhary, “Benzophenone Photoprobes for Phosphoinositides, Peptides, and Drugs” Photochem. Photobiol. 65: 222-234 (1997).

    A. Chaudhary and G.D. Prestwich, “Adenophostins:  Newly Discovered Metabolites ofPenicillium brevicompactum as Potent Agonists of the Inositol 1,4,5 Trisphosphate Receptor” ChemTractsOrg. Chem. 7: 111-114 (1994).